Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659807.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G
HEPES, Free Acid is a biochemical reagent used for maintaining a stable pH environment in biological and chemical applications. This 250g package is ideal for preparing buffered solutions for use in cell culture, protein analysis, and other biochemical experiments. HEPES, or 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, is widely used because of its excellent buffering capacity in the pH range of 6.8 to 8.2. It is commonly used in cell biology to maintain physiological pH during experiments. This reagent is highly stable, non-toxic to cells, and is ideal for applications that require precise pH control over extended periods of time.
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660276.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg
HEPES, Free Acid is a powerful biochemical reagent provided in a 5kg size, ideal for large-scale applications in biological and biochemical research. It helps maintain a stable pH in the range of 6.8–8.2, which is critical for a variety of assays, including cell culture, protein purification, and enzyme assays. The reagent's excellent buffering capacity ensures that pH remains stable throughout experiments, preventing fluctuations that could affect results. Its low toxicity and high stability make it particularly useful in biological systems, offering consistent performance and reproducible results for laboratories involved in cell-based studies and biochemical analysis.
Alkali Scientific ADA, [N (Carbamoylmethyl) iminodiacetic acid), 100 G
ADA (N-(Carbamoylmethyl) iminodiacetic acid) is a biochemical reagent available in a 100g bottle. This buffering agent is commonly used in laboratory settings for maintaining a stable pH in aqueous solutions. ADA is often employed in chromatography, enzyme assays, and protein purification where precise pH control is essential. It works effectively in various biochemical and molecular biology applications, providing a stable environment for enzymes and proteins. The 100g size is ideal for research laboratories and industrial applications requiring high-quality reagents for pH regulation and buffering in biochemical processes.
ILEX LIFE SCIENCES LLC PAN Biotech HEPES Buffer 1 M GMP Grade Cell Culture Buffer 100 ML 1/EA
PAN-Biotech HEPES Buffer 1 M GMP Grade 100 ml INTRODUCTION HEPES is a zwitterionic sulfonic acid buffering agent widely used in cell culture largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers GMP GRADE PAN-Biotech HEPES Buffer 1 M GMP Grade is a ready-to-use highly concentrated buffer solution designed for direct use in GMP-regulated clinical and industrial applications The GMP grade version is based on the proven HEPES formulation and is manufactured under strictly controlled GMP conditions Defined raw materials high batch-to-batch consistency and comprehensive documentation make HEPES Buffer 1 M GMP a true ready-to-use solution suitable for immediate use in sensitive cell culture and manufacturing workflows COMPOSITION HEPES 238 31 g/L QUALITY GMP Grade (EU Annex 1) LIQUID/POWDER Liquid STERILE Yes STORAGE 2-8 C
Medchemexpress LLC 4-Morpholineethanesulfonic acid, potassium salt | 39946-25-3 | 99.9% | 10 G
MES potassium (2-Morpholinoethanesulphonic acid potassium) is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. As a Good's buffer, it is utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15 and shows poor affinity with metal ions, making it suitable for studies involving them.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at around pH 6.15
- Poor affinity with metal ions, making it suitable for studies involving metal ions
![Alkali Scientific MOPSO [3 (N Morpholino) 2 hydroxypropanesulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661261.jpg-250.jpg)
Alkali Scientific MOPSO [3 (N Morpholino) 2 hydroxypropanesulfonic acid], 25 G
MOPSO (3-(N-Morpholino)2-hydroxypropanesulfonic acid) sodium salt is a pH buffering reagent used in biochemical and biological applications requiring stable pH control. This 25g volume of MOPSO sodium salt is ideal for laboratories with specific buffer preparation needs for enzyme assays, protein studies, and other research applications. MOPSO is widely used in molecular biology protocols, including nucleic acid analysis, where maintaining a constant pH of 7.0–7.5 is critical. Its buffering capacity helps maintain pH stability during reactions and experiments, ensuring optimal conditions for enzymatic activity and other biochemical processes. MOPSO is a valuable reagent in research involving protein, DNA, and RNA studies.
Alkali Scientific Bicine Ultra Pure, 250 G
Bicine Ultra Pure, available in a 250g package, is a high-quality buffer ideal for a range of biochemical and molecular biology applications. This buffer provides excellent pH stability in the range of 7.6–9.0 and is widely used in protein purification, electrophoresis, and enzyme assays. Its low ionic strength and minimal background interference make it particularly valuable for sensitive applications where precise pH control is necessary. The 250g size is ideal for medium-scale laboratory operations, providing an efficient supply for research projects and experiments requiring reliable buffering capacity over extended periods. Bicine Ultra Pure is a critical reagent in proteomics and genomics research.